The sterilization methods of glassware
Nov 15, 2024

The sterilization methods of glassware mainly include hot and wet sterilization, dry heat sterilization and chemical sterilization.
Hot and humid sterilization
Hot wet sterilization is a common and effective sterilization method, which is suitable for heat-resistant glassware. The steps are as follows:
Place the glassware that needs to be sterilized in a sterilizer (also known as a high-pressure steam sterilizer or pressure cooker).
Set the appropriate sterilization temperature and pressure parameters, usually 121 degrees Celsius with a pressure of 15psi (pound force per square inch).
Sterilization time depends on the size of the vessel and sterilization requirements, usually 15-30 minutes.
After sterilization is complete, wait for the container to cool to a safe temperature before taking it out to avoid burns.
Dry heat sterilization
Dry heat sterilization is the sterilization of utensils by high temperature dry heat, which is suitable for some heat-resistant glassware. The steps are as follows:
Place the glassware to be sterilized in a dry heat sterilizer.
Set the appropriate sterilization temperature and time parameters, usually 160-180 degrees Celsius, for 1-2 hours.
After sterilization, wait until the container has cooled to a safe temperature and take it out for use.
Chemical sterilization
Chemical sterilization The use of chemical disinfectants to sterilize utensils, suitable for some special requirements or do not withstand high temperature glassware. The steps are as follows:
Choose the right chemical disinfectants, such as formaldehyde, peracetic acid, etc.
Soak the utensil in a chemical disinfectant solution and sterilize it according to the specified soaking time.
After sterilization is complete, wash the utensils thoroughly with pure water or other appropriate solvents to remove residual disinfectant.
Note 1:
Before sterilization, be sure to clean the surface of the glassware to remove residue and dirt to ensure sterilization effect.
For glassware used in laboratories, it is best to use vessels that are resistant to high temperature and pressure to ensure the sterilization effect and the safety of the vessels.
Select appropriate sterilization methods and parameters according to specific needs and experimental requirements to ensure that the sterilization effect meets the requirements.
